Molecular identification of a Drosophila G protein-coupled receptor specific for crustacean cardioactive peptide

被引：47

作者：

Cazzamali, G ^{[1
]}

Hauser, F ^{[1
]}

Kobberup, S ^{[1
]}

Williamson, M ^{[1
]}

Grimmelikhuijzen, CJP ^{[1
]}

机构：

[1] Univ Copenhagen, Inst Zool, Dept Cell Biol, DK-2100 Copenhagen, Denmark

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 303卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(03)00302-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Drosophila Genome Project website (www.flybase.org) contains the sequence of an annotated gene (CG6111) expected to code for a G protein-coupled receptor. We have cloned this receptor and found that its gene was not correctly predicted, because an annotated neighbouring gene (CG14547) was also part of the receptor gene. DNA corresponding to the corrected gene CG6111 was expressed in Chinese hamster ovary cells, where it was found to code for a receptor that could be activated by low concentrations of crustacean cardioactive peptide, which is a neuropeptide also known to occur in Drosophila and other insects (EC50, 5.4 x 10(-10) M). Other known Drosophila neuropeptides, such as adipokinetic hormone, did not activate the receptor. The receptor is expressed in all developmental stages from Drosophila, but only very weakly in larvae. In adult flies, the receptor is mainly expressed in the head. Furthermore, we identified a gene sequence in the genomic database from the malaria mosquito Anopheles gambiae that very likely codes for a crustacean cardioactive peptide receptor. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：146 / 152

页数：7

共 26 条

[1] The genome sequence of Drosophila melanogaster [J].

Adams, MD ;

Celniker, SE ;

Holt, RA ;

Evans, CA ;

Gocayne, JD ;

Amanatides, PG ;

Scherer, SE ;

Li, PW ;

Hoskins, RA ;

Galle, RF ;

George, RA ;

Lewis, SE ;

Richards, S ;

Ashburner, M ;

Henderson, SN ;

Sutton, GG ;

Wortman, JR ;

Yandell, MD ;

Zhang, Q ;

Chen, LX ;

Brandon, RC ;

Rogers, YHC ;

Blazej, RG ;

Champe, M ;

Pfeiffer, BD ;

Wan, KH ;

Doyle, C ;

Baxter, EG ;

Helt, G ;

Nelson, CR ;

Miklos, GLG ;

Abril, JF ;

Agbayani, A ;

An, HJ ;

Andrews-Pfannkoch, C ;

Baldwin, D ;

Ballew, RM ;

Basu, A ;

Baxendale, J ;

Bayraktaroglu, L ;

Beasley, EM ;

Beeson, KY ;

Benos, PV ;

Berman, BP ;

Bhandari, D ;

Bolshakov, S ;

Borkova, D ;

Botchan, MR ;

Bouck, J ;

Brokstein, P .

SCIENCE, 2000, 287 (5461) :2185-2195

[2] Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster [J].

Broeck, JV .

PEPTIDES, 2001, 22 (02) :241-254

[3]

Budd G E, 2001, Science, V294, P2047

[4] Molecular cloning and functional expression of a Drosophila corazonin receptor [J].

Cazzamali, G ;

Saxild, NPE ;

Grimmelikhuijzen, CJP .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 298 (01) :31-36

[5] Molecular cloning and functional expression of the first insect FMRFamide receptor [J].

Cazzamali, G ;

Grimmelikhuijzen, CJP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (19) :12073-12078

[6] ISOLATION AND IDENTIFICATION OF A CARDIOACTIVE PEPTIDE FROM TENEBRIO-MOLITOR AND SPODOPTERA-ERIDANIA [J].

FURUYA, K ;

LIAO, S ;

REYNOLDS, SE ;

OTA, RB ;

HACKETT, M ;

SCHOOLEY, DA .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1993, 374 (12) :1065-1074

[7] Hormonal regulation in insects: Facts, gaps, and future directions [J].

Gade, G ;

Hoffmann, KH ;

Spring, JH .

PHYSIOLOGICAL REVIEWS, 1997, 77 (04) :963-1032

[8]

Gammie SC, 1999, J EXP BIOL, V202, P343

[9] Molecular cloning, genomic organization and developmental regulation of a novel receptor from Drosophila melanogaster structurally related to gonadotropin-releasing hormone receptors from vertebrates [J].

Hauser, F ;

Sondergaard, L ;

Grimmelikhuijzen, CJP .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 249 (03) :822-828

[10] The genome sequence of the malaria mosquito Anopheles gambiae [J].

Holt, RA ;

Subramanian, GM ;

Halpern, A ;

Sutton, GG ;

Charlab, R ;

Nusskern, DR ;

Wincker, P ;

Clark, AG ;

Ribeiro, JMC ;

Wides, R ;

Salzberg, SL ;

Loftus, B ;

Yandell, M ;

Majoros, WH ;

Rusch, DB ;

Lai, ZW ;

Kraft, CL ;

Abril, JF ;

Anthouard, V ;

Arensburger, P ;

Atkinson, PW ;

Baden, H ;

de Berardinis, V ;

Baldwin, D ;

Benes, V ;

Biedler, J ;

Blass, C ;

Bolanos, R ;

Boscus, D ;

Barnstead, M ;

Cai, S ;

Center, A ;

Chatuverdi, K ;

Christophides, GK ;

Chrystal, MA ;

Clamp, M ;

Cravchik, A ;

Curwen, V ;

Dana, A ;

Delcher, A ;

Dew, I ;

Evans, CA ;

Flanigan, M ;

Grundschober-Freimoser, A ;

Friedli, L ;

Gu, ZP ;

Guan, P ;

Guigo, R ;

Hillenmeyer, ME ;

Hladun, SL .

SCIENCE, 2002, 298 (5591) :129-+

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