Studies on the diversity of the distinct phylogenetic lineage encompassing Glomus claroideum and Glomus etunicatum

被引:17
作者
Rodriguez, A
Clapp, JP
Robinson, L
Dodd, JC
机构
[1] Univ Kent, Res Sch Biosci, Canterbury CT2 7NJ, Kent, England
[2] Sittingbourne Res Ctr, Int Inst Biotechnol, Sittingbourne ME9 8HL, Kent, England
关键词
Glomus claroideum/Glomus etunicatum clade; rRNA large subunit; multiple sequences; gene sequence diversity;
D O I
10.1007/s00572-003-0291-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Morphological and molecular characters were analysed to investigate diversity within isolates of the Glomus claroideum/Glomus etunicatum species group in the genus Glomus. The inter- and intra-isolate sequence diversity of the large subunit (LSU) rRNA gene D2 region of eight isolates of G. claroideum and G. etunicatum was studied using PCR-single strand conformational polymorphism (SSCP)-sequencing. In addition, two isolates recently obtained from Southern China were included in the analysis to allow for a wider geographic screening. Single spore DNA isolation confirmed the magnitude of gene diversity found in multispore DNA extractions. An apparent overlap of spore morphological characters was found between G. claroideum and G. etunicatum in some isolates. Analysis of the sequence frequencies in all G. etunicatum and G. claroideum isolates (ten) showed that four LSU D2 sequences, representing 32.1% of the clones analysed for multispore extraction (564) were found to be common to both species, and those sequences were the most abundant in four of the ten isolates analysed. The frequency of these sequences ranged between 23.2% and 87.5% of the clones analysed in each isolate. The implications for the use of phenotypic characters to define species in arbuscular mycorrhizal fungi are discussed. The current position of G. claroideum/G.etunicatum in the taxonomy of the Glomeromycota is also discussed.
引用
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页码:33 / 46
页数:14
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