Protein kinase C-α participates in FcγR-mediated phagocytosis in macrophages

被引：43

作者：

Breton, A ^{[1
]}

Descoteaux, A ^{[1
]}

机构：

[1] Univ Quebec, Inst Armand Frappier, INRS, Laval, PQ H7V 1B7, Canada

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 276卷 / 02期

基金：

英国医学研究理事会;

关键词：

phagocytosis; macrophage; Fc receptor; protein kinase C; signal transduction; ERK1/2;

D O I：

10.1006/bbrc.2000.3511

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phagocytosis of IgG-opsonized particles by macrophages requires the activation of protein kinase C (PKC), a family of protein serine/threonine kinases. In the present study, we have investigated the role of the PKC-alpha isoenzyme in Fc gamma R-mediated phagocytosis using clones of the mouse macrophage cell line RAW 264.7 overexpressing a dominant-negative (DN) mutant of PKC-alpha. Overexpression of DN PKC-alpha had no effect on the attachment of IgG-opsonized sheep red blood cells, but inhibited their internalization. Further analysis of the signaling events induced by IgG-opsonized sheep red blood cells revealed that whereas tyrosine phosphorylation of Syk was normal, phosphorylation of ERK 1/2 (p42/44) was impaired in DN PRC-alpha-overexpressing macrophages. These observations suggest a role for PKC-alpha in the regulation of Fc gamma R-induced phagocytosis and signal transduction. (C) 2000 Academic Press.

引用

页码：472 / 476

页数：5

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