Measurement of the kinetic isotope effect for the oxidation of NADH at a poly(aniline)-modified electrode

被引:36
作者
Bartlett, PN [1 ]
Simon, E [1 ]
机构
[1] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England
关键词
D O I
10.1021/ja028943e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Kinetic isotope measurements using [4,4-2H2]NADH and [4-1H, 4-2H]NADH have been used to investigate the mechanism of the electrochemical oxidation of NADH at poly(aniline)-poly(vinyl sulfonate)-modified electrodes. The experiments show a primary kinetic isotope effect for the reaction of 4.2. This is consistent with literature values for the corresponding isotope effect for the oxidation of NADH by two-electron oxidants in homogeneous solution. The result demonstrates that transfer of H from NADH to the modified electrode occurs in the rate-limiting step within the reaction complex. Copyright © 2003 American Chemical Society.
引用
收藏
页码:4014 / 4015
页数:2
相关论文
共 12 条