Relaxation of rat distal colon by activation of muscarinic, neuronal receptors:: possible involvement of P2y-purinoceptors

被引:6
作者
Börjesson, L [1 ]
Ali, A [1 ]
Nordgren, S [1 ]
Delbro, DS [1 ]
机构
[1] Sahlgrenska Univ Hosp, Dept Surg, Inst Surg Sci, S-41345 Gothenburg, Sweden
来源
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM | 2000年 / 81卷 / 1-3期
关键词
colon; enteric nervous system; McN-A-343; muscarinic receptor; nitric oxide; non-adrenergic relaxation; P-2y-purinoceptor; purinergic; smooth muscle;
D O I
10.1016/S0165-1838(00)00112-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
McN-A-343, which is a ligand at muscarinic receptors on myenteric ganglia, was found to concentration-dependently (1-44 mu M) elicit non-adrenergic relaxation of the longitudinal muscle of rat distal colon, having been precontracted with carbachol (1 mu M). This effect was partly antagonized by the muscarinic receptor antagonist, pirenzepine (0.3 mu M), the nerve blocker, tetrodotoxin (1 mu M), or by drugs which interfere with purinergic neurotransmission (apamin [0.5 mu M], reactive blue 2 [50 mu M]). Blockade of nitric oxide synthase (L-NNA [100 mu M]), or of the cAMP (H-89 [1 mu M]), or cGMP (ODQ [10 mu M]) second messenger pathways did not affect the relaxatory response to McN-A-343 (14 mu M). An additional, non-neurogenic component of the relaxation to this compound on carbachol induced tone is suggested to reflect a partial antagonism of the muscarinic receptors on the gut muscle by McN-A-343. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:53 / 58
页数:6
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