The effect of mannan-binding lectin on opsonophagocytosis of Neisseria meningitidis

被引:16
作者
Drogari-Apiranthitou, M
Fijen, CAP
Thiel, S
Platonov, A
Jensen, L
Dankert, J
Kuijper, EJ
机构
[1] Univ Amsterdam, Dept Med Microbiol, NL-1105 AZ Amsterdam, Netherlands
[2] Aarhus Univ, Inst Med Microbiol, DK-8000 Aarhus, Denmark
[3] Inst Epidemiol, Moscow 111123, Russia
来源
IMMUNOPHARMACOLOGY | 1997年 / 38卷 / 1-2期
关键词
mannan-binding lectin; opsonophagocytosis; Neisseria meningitidis;
D O I
10.1016/S0162-3109(97)00081-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mannan-binding lectin (MEL), an acute phase protein with a structure and a function very similar to that of Clq, is known to act as an opsonin binding to a number of microorganisms. In order to investigate the effect of MBL on the phagocytic killing of meningococci, a serogroup B meningococcal strain (H44/76) and its unencapsulated variant v24, as well as a serogroup A meningococcal strain were opsonized with MBL (purified from normal human plasma at the State Serum Institute, Denmark) and used in a phagocytic killing assay at a density of 7 x 10(3) CFU/ml. Polymorphonuclear cells (PMNs) from one healthy donor were isolated by density gradient centrifugation over Percoll and added to the system (7 x 10(6) cells/ml). In a first set of experiments without addition of serum or complement, no influence of MBL was observed on the killing of any of these strains. Addition of MBL to non-opsonized bacteria of the serogroup A strain did not result in enhanced killing either; on the contrary, the growth of this strain increased significantly when a high MBL concentration (40 mu g/ml) was used in the presence of PMNs. Further investigations were performed using sera of five individuals with late complement component deficiency (LCCD) and a concomitant MBL deficiency, vaccinated with a tetra-valent (ACYW135) meningococcal capsular polysaccharide vaccine. Pre-and post-vaccination sera (50% final concentration) were tested against a group A strain opsonized or not with MEL. In only one patient was there a moderate increase of killing of the opsonized bacteria after vaccination compared to pre-vaccination serum. Our results suggest that MBL may not play a significant role in the opsonophagocytosis of meningococci, irrespective of its binding to unencapsulated and serogroup A strains. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:93 / 99
页数:7
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