Production of L-DOPA from tyrosinase immobilized on nylon 6,6: enzyme stability and scaleup

被引:51
作者
Pialis, P [1 ]
Saville, BA [1 ]
机构
[1] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
L-DOPA; tyrosinase; scaleup; kinetics; inactivation;
D O I
10.1016/S0141-0229(97)00195-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of L-3,4 dihydroxyphenylalanine (L-DOPA) from mushroom tyrosinase immobilized on chemically modified nylon 6,6 membranes was investigated in a batch reactor. The effects of product inhibition, oxygen partial pressure, and scaleup upon L-DOPA production rates and tyrosinase activity were studied L-DOPA production rates were strongly influenced by oxygen and L-DOPA concentrations. Kinetic modeling revealed that L-DOPA concentrations as low as 1 mM would reduce the L-DOPA production rate by approximately 50% due to competitive inhibition. The average L-DOPA production rate increased by 10% when the oxygen partial pressure was increased from 21 kPa and 100 kPa; however, at higher oxygen partial pressures (50 kPa and 100 kPa), the rare of oxidative inactivation of tyrosinase increased causing the enzyme half-life to decrease from 46 h under 21 kPa oxygen to 7.7 h under 100 kPa oxygen. The turnover number was approximately 1,170 under 21 kPa oxygen, but dropped to approximately 150 under 100 kPa oxygen. Scaleup from a 500-ml batch reactor to 1-l and 2-l reactors proved to be straightforward. Essentially identical production rates were obtained as long as the enzyme concentration within the reactor was held constant. If the enzyme concentration was varied, a proportional change in the L-DOPA production rate was not observed due to the effects of product inhibition. (C) 1998 Elsevier Science Inc.
引用
收藏
页码:261 / 268
页数:8
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