Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei

被引:364
作者
Godoy, D
Randle, G
Simpson, AJ
Aanensen, DM
Pitt, TL
Kinoshita, R
Spratt, BG
机构
[1] Univ London Imperial Coll Sci Technol & Med, St Marys Hosp, Dept Infect Dis Epidemiol, Fac Med,Sch Med, London W2 1PG, England
[2] John Radcliffe Hosp, Weatherall Inst Mol Med, Mol Infect Dis Grp, Oxford OX3 9DS, England
[3] Royal Free Hosp, Dept Med Microbiol, London NW3 2QG, England
[4] Cent Publ Hlth Lab, Lab Hosp Infect, London NW9 5HT, England
[5] Univ Hong Kong, Fac Med, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
[6] Univ Hong Kong, Fac Med, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[7] Ocean Pk Corp, Vet Hosp, Hong Kong, Hong Kong, Peoples R China
基金
英国惠康基金;
关键词
D O I
10.1128/JCM.41.5.2068-2079.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A collection of 147 isolates of Burkholderia pseudomallei, B. mallei, and B. thailandensis was characterized by multilocus sequence typing (MLST). The 128 isolates of B. pseudomallei, the causative agent of melioidosis, were obtained from diverse geographic locations, from humans and animals with disease, and from the environment and were resolved into 71 sequence types. The utility of the MLST scheme for epidemiological investigations was established by analyzing isolates from captive marine mammals and birds and from humans in Hong Kong with melioidosis. MLST gave a level of resolution similar to that given by pulsed-field get electrophoresis and identified the same three clones causing disease in animals, each of which was also associated with disease in humans. The average divergence between the alleles of B. thailandensis and B. pseudomallei was 3.2%, and there was no sharing of alleles between these species. Trees constructed from differences in the allelic profiles of the isolates and from the concatenated sequences of the seven loci showed that the B. pseudomallei isolates formed a cluster of closely related lineages that were fully resolved from the cluster of B. thailandensis isolates, confirming their separate species status. However, isolates of B. mallei, the causative agent of glanders, recovered from three continents over a 30-year period had identical allelic profiles, and the B. mallei isolates clustered within the B. pseudomallei group of isolates. Alleles at six of the seven loci in B. mallei were also present within B. pseudomallei isolates, and B. mallei is a clone of B. pseudomallei that, on population genetics grounds, should not be given separate species status.
引用
收藏
页码:2068 / 2079
页数:12
相关论文
共 26 条
[1]   Mother-to-child transmission of Burkholderia pseudomallei. [J].
Abbink, FC ;
Orendi, JM ;
de Beaufort, AJ .
NEW ENGLAND JOURNAL OF MEDICINE, 2001, 344 (15) :1171-1172
[2]   Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis [J].
Achtman, M ;
Zurth, K ;
Morelli, C ;
Torrea, G ;
Guiyoule, A ;
Carniel, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) :14043-14048
[3]   Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains [J].
Brett, PJ ;
Deshazer, D ;
Woods, DE .
EPIDEMIOLOGY AND INFECTION, 1997, 118 (02) :137-148
[4]   Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species [J].
Brett, PJ ;
DeShazer, D ;
Woods, DE .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :317-320
[5]   A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates [J].
Currie, BJ ;
Mayo, M ;
Anstey, NM ;
Donohoe, P ;
Haase, A ;
Kemp, DJ .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2001, 65 (03) :177-179
[6]   Melioidosis [J].
Dance, DAB .
CURRENT OPINION IN INFECTIOUS DISEASES, 2002, 15 (02) :127-132
[7]   Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus [J].
Enright, MC ;
Day, NPJ ;
Davies, CE ;
Peacock, SJ ;
Spratt, BG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) :1008-1015
[8]   A multilocus sequence typing scheme for Streptococcus pneumoniae:: identification of clones associated with serious invasive disease [J].
Enright, MC ;
Spratt, BG .
MICROBIOLOGY-UK, 1998, 144 :3049-3060
[9]   SUBDIVISION OF BURKHOLDERIA-PSEUDOMALLEI RIBOTYPES INTO MULTIPLE TYPES BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS PROVIDES NEW INSIGHTS INTO EPIDEMIOLOGY [J].
HAASE, A ;
SMITHVAUGHAN, H ;
MELDER, A ;
WOOD, Y ;
JANMAAT, A ;
GILFEDDER, J ;
KEMP, D ;
CURRIE, B .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) :1687-1690
[10]  
HOWE C, 1950, OXFORD MED, P185