Synthesis and characterization of a sulfhydryl-reactive rhenium metal-ligand complex

被引：49

作者：

Dattelbaum, JD ^{[1
]}

Abugo, OO ^{[1
]}

Lakowicz, JR ^{[1
]}

机构：

[1] Univ Maryland, Sch Med, Ctr Fluorescence Spect, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA

来源：

BIOCONJUGATE CHEMISTRY | 2000年 / 11卷 / 04期

关键词：

D O I：

10.1021/bc990174+

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the synthesis and spectral characterization of a rhenium metal-ligand complex. This complex reacts with sulfhydryl groups via an iodoacetamide side chain on the phenanthroline ligand and displays a high limiting anisotropy near 0.35 when excited at 442 nm. When covalently linked to human serum albumin, this complex displayed a mean decay time of about 1 mu s. This decay time is appropriate for measuring rotational correlation times on the microsecond time scale as may occur for large macromolecular complexes.

引用

页码：533 / 536

页数：4

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