Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis

被引：253

作者：

Bai, Lingling ^{[1
,2
]}

Shao, Hui ^{[3
]}

Wang, Hongxing ^{[4
]}

Zhang, Zhihui ^{[1
,2
]}

Su, Chang ^{[1
,2
]}

Dong, Lijie ^{[1
,2
]}

Yu, Bo ^{[1
,2
]}

Chen, Xiteng ^{[1
,2
]}

Li, Xiaorong ^{[1
,2
]}

Zhang, Xiaomin ^{[1
,2
]}

机构：

[1] Tianjin Med Univ, Eye Hosp, Eye Inst, Tianjin 300384, Peoples R China

[2] Tianjin Med Univ, Eye Hosp, Sch Optometry & Ophthalmol, Tianjin 300384, Peoples R China

[3] Univ Louisville, Kentucky Lions Eye Ctr, Dept Ophthalmol & Visual Sci, Louisville, KY 40202 USA

[4] Chuiyangliu Hosp, Dept Ophthalmol, Beijing, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

EXTRACELLULAR VESICLES; THERAPY; IMPROVE; INJURY;

D O I：

10.1038/s41598-017-04559-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. Recently, MSC-derived exosomes (MSC-Exo) were thought to carry functions of MSCs. In this study, we tested the effect of local administration of human MSC-Exo on established EAU in the same species. Rats with EAU induced by immunization with interphotoreceptor retinol-binding protein 1177-1191 peptide were treated by periocular injections of increasing doses of MSC-Exo starting at the disease onset for 7 consecutive days. The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. We found that MSC-Exo greatly reduced the intensity of ongoing EAU as their parent cells by reducing the infiltration of T cell subsets, and other inflammatory cells, in the eyes. Furthermore, the chemoattractive effects of CCL2 and CCL21 on inflammatory cells were inhibited by MSC-Exo. However, no inhibitory effect of MSC-Exo on IRBP-specific T cell proliferation was observed. These results suggest that MSC-Exo effectively ameliorate EAU by inhibiting the migration of inflammatory cells, indicating a potential novel therapy of MSC-Exo for uveitis.

引用

页数：11

共 42 条

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