Generation of resistance to the Diphenyl ether herbicide, oxyfluorfen, via expression of the Bacillus subtilis protoporphyrinogen oxidase gene in transgenic tobacco plants

被引:34
作者
Choi, KW
Han, O [1 ]
Lee, HJ
Yun, YC
Moon, YH
Kim, M
Kuk, YI
Han, SU
Guh, JO
机构
[1] Chonnam Natl Univ, Inst Agr Sci & Technol, Kwangju 500757, South Korea
[2] Jinro Cent Res Inst, Plant Biotechnol Lab, Yongin 449910, South Korea
[3] Chonnam Natl Univ, Inst Biotechnol, Kwangju 500757, South Korea
[4] Chonnam Natl Univ, Dept Agron, Kwangju 500757, South Korea
关键词
diphenyl ether herbicide; protoporphyrinogen oxidase; Bacillus subtilis; oxyfluorfen; transgenic tobacco plant;
D O I
10.1271/bbb.62.558
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tn an effort to develop transgenic plants resistant to diphenyl ether herbicides, vee introduced the protoporphyrinogen oxidase (EC 1.3.3.4) gene of Bacillus subtilis into tobacco plants, The results from a Northern analysis and leaf disc assay indicate that the expression of the B. subtilis protoporphyrinogen oxidase gene under the cauliflower mosaic virus 35S promoter generated resistance to the diphenyl ether herbicide, oxyfluorfen, in transgenic tobacco plants.
引用
收藏
页码:558 / 560
页数:3
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