Short report: Development of a new diagnostic method for Plasmodium falciparum infection using a reverse transcriptase-polymerase chain reaction

被引：8

作者：

Abdullah, NR ^{[1
]}

Furuta, T ^{[1
]}

Taib, R ^{[1
]}

Kita, K ^{[1
]}

Kojima, S ^{[1
]}

Wah, MJ ^{[1
]}

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT PARASITOL,MINATO KU,TOKYO 108,JAPAN

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1996年 / 54卷 / 02期

关键词：

D O I：

10.4269/ajtmh.1996.54.162

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We describe here a reverse transcriptase-polymerase chain reaction method for the detection of malaria parasites. Ten in vitro-cultured isolates of Plasmodium falciparum and 16 specimens from patients infected with P. falciparum were used to examine the specificity and sensitivity of the test. The sensitivity of the test was 0.3 parasites per microliter of blood. Specificity was determined by matching the sequences of the specimens' DNA to published sequences of 18S ribosomal RNA genes in the species-specific region. The test proved to be very sensitive and specific for the detection of P. falciparum infection.

引用

页码：162 / 163

页数：2

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