Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation

被引：148

作者：

Brandt, Dominique T.

Marion, Sabrina

Griffiths, Gareth

Watanabe, Takashi

Kaibuchi, Kozo

Grosse, Robert ^{[1
]}

机构：

[1] Heidelberg Univ, Inst Pharmacol, D-69120 Heidelberg, Germany

[2] European Mol Biol Lab, D-69117 Heidelberg, Germany

[3] Nagoya Univ, Grad Sch Med, Dept Cell Pharmacol, Nagoya, Aichi 466, Japan

来源：

JOURNAL OF CELL BIOLOGY | 2007年 / 178卷 / 02期

关键词：

D O I：

10.1083/jcb.200612071

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

The Diaphanous-related formin Dial nucleates actin polymerization, thereby regulating cell shape and motility. Mechanisms that control the cellular location of Dial to spatially define actin polymerization are largely unknown. In this study, we identify the cytoskeletal scaffold protein IQGAP1 as a Dial-binding protein that is necessary for its subcellular location. IQGAP1 interacts with Dial through a region within the Diaphanous inhibitory domain after the RhoA-mediated release of Dial autoinhibition. Both proteins colocalize at the front of migrating cells but also at the actin-rich phagocytic cup in macrophages. We show that IQGAP1 interaction with Dial is required for phagocytosis and phagocytic cup formation. Thus, we identify IQGAP1 as a novel component involved in the regulation of phagocytosis by mediating the localization of the actin filament nucleator Dial.

引用

页码：193 / 200

页数：8

共 26 条

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