Chronic lithium treatment decreases brain phospholipase A2 activity

被引：68

作者：

Chang, MCJ ^{[1
]}

Jones, CR ^{[1
]}

机构：

[1] NIA, Bethesda, MD 20892 USA

来源：

NEUROCHEMICAL RESEARCH | 1998年 / 23卷 / 06期

关键词：

lithium; phospholipase A(2); arachidonate; brain; phospholipids; phosphatidylcholine;

D O I：

10.1023/A:1022415113421

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chronic lithium administration decreases the turnover of arachidonic acid (AA) in several brain phospholipids. This suggests that lithium may attenuate phospholipase A(2) (PLA(2)) activity in brain. We now report effects of chronic lithium treatment on PLA(2) activity in postnuclear supernatant from rat brain: Enzyme activity was determined by two assay methods, radiometric and fluorometric, and measured the release of the fatty acid on the second acyl position (sn2) from choline and ethanolamine phospholipids. PLA(2) activity in brain postnuclear supernatant from rats chronically treated with lithium in the diet was significantly decreased (20-50%) when compared with controls. In vehicle or lithium-treated rats, PLA(2) activity was not significantly augmented or attenuated by the addition of calcium chelators, divalent cations or LiCl supplementation (1.0 mM) to postnuclear supernatant. These results suggest that a major therapeutic effect of lithium is to attenuate brain PLA(2) activity involved in signal transduction.

引用

页码：887 / 892

页数：6

共 44 条

[1] SOME ASPECTS OF RAT PLATELET AND SERUM PHOSPHOLIPASE-A2 ACTIVITIES [J].