Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain

被引：106

作者：

Akke, M

Liu, J

Cavanagh, J

Erickson, HP

Palmer, AG ^{[1
]}

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[2] Genentech Inc, Pharmacol Res & Dev, S San Francisco, CA 94080 USA

[3] New York State Dept Hlth, Wadsworth Ctr Labs & Res, NMR Struct Biol Facil, Albany, NY 12201 USA

[4] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1998年 / 5卷 / 01期

关键词：

D O I：

10.1038/nsb0198-55

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel off-resonance rotating-frame N-15 NMR spin relaxation experiment is used to characterize conformational fluctuations with correlation times between 32 and 175 mu s in the third fibronectin type III domain of human tenascin-C. Conformational fluctuations of contiguous regions of the beta-sandwich structure of the type III domain may represent collective motions, such as transient twisting or breathing of the beta-sheets. Flexibility of the loop containing the Arg-Gly-Asp (RGD) tripeptide may affect the accessibility of this motif in protein-protein interactions.

引用

页码：55 / 59

页数：5

共 46 条

[1] HETERONUCLEAR-CORRELATION NMR-SPECTROSCOPY WITH SIMULTANEOUS ISOTOPE FILTRATION, QUADRATURE DETECTION, AND SENSITIVITY ENHANCEMENT USING Z-ROTATIONS [J].