Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain

被引:106
作者
Akke, M
Liu, J
Cavanagh, J
Erickson, HP
Palmer, AG [1 ]
机构
[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA
[2] Genentech Inc, Pharmacol Res & Dev, S San Francisco, CA 94080 USA
[3] New York State Dept Hlth, Wadsworth Ctr Labs & Res, NMR Struct Biol Facil, Albany, NY 12201 USA
[4] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
关键词
D O I
10.1038/nsb0198-55
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel off-resonance rotating-frame N-15 NMR spin relaxation experiment is used to characterize conformational fluctuations with correlation times between 32 and 175 mu s in the third fibronectin type III domain of human tenascin-C. Conformational fluctuations of contiguous regions of the beta-sandwich structure of the type III domain may represent collective motions, such as transient twisting or breathing of the beta-sheets. Flexibility of the loop containing the Arg-Gly-Asp (RGD) tripeptide may affect the accessibility of this motif in protein-protein interactions.
引用
收藏
页码:55 / 59
页数:5
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