Rapid identification of the genus Fonsecaea by PCR with specific oligonucleotide primers

被引：38

作者：

Abliz, P ^{[1
]}

Fukushima, K ^{[1
]}

Takizawa, K ^{[1
]}

Nieda, N ^{[1
]}

Miyaji, M ^{[1
]}

Nishimura, K ^{[1
]}

机构：

[1] Chiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Chuo Ku, Chiba 2608673, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 02期

关键词：

D O I：

10.1128/JCM.41.2.873-876.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 by for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus Fonsecaea.

引用

页码：873 / 876

页数：4

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