L-Lysine catabolism is osmo-regulated at the level of lysine-ketoglutarate reductase and saccharopine dehydrogenase in rapeseed leaf discs

We have previously shown that a gene encoding for lysine-ketoglutarate reductase (LKR, EC 1.5.1.8) and saccharopine dehydrogenase (SDH, EC 1.5.1.9) is upregulated in osmotically stressed leaf discs from Brassica napus. In plants, excess lysine is catabolised by these enzymes which are linked on a single polypeptide. These findings suggested that LKR and SDH activities could be enhanced with decreasing osmotic potential. This proposal has been assessed in this study where LKR and SDH activities were determined in desalted crude extracts from rapeseed leaf discs subjected in vitro to upshock osmotic stress using polyethylene glycol (PEG) as a non-permeant osmoticum. Results reported here demonstrated that LKR and SDH activities increased in stressed material similarly to that observed for the related mRNA levels. In addition, it was shown that both activities depend on the intensity of the external osmotic stress and the duration of the applied treatment. On the other hand, during recovery of leaf discs upshocked and then downshocked, LKR and SDH activities decreased which clearly demonstrated that lysine catabolism is osmo-regulated through these activities. (C) 2000 Editions scientifiques et medicales Elsevier SAS.