Hybrid respiration in the denitrifying mitochondria of Fusarium oxysporum

被引：36

作者：

Takaya, N

Kuwazaki, S

Adachi, Y

Suzuki, S

Kikuchi, T

Nakamura, H

Shiro, Y

Shoun, H ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058572, Japan

[2] RIKEN, Harima Inst, Inst Phys & Chem Res Inst, Sayo, Hyogo 6795143, Japan

[3] Univ Tokyo, Dept Biotechnol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2003年 / 133卷 / 04期

关键词：

aerobic respiration; cytochrome c; Fusarium oxysporum; mitochondrion; nitrate respiration;

D O I：

10.1093/jb/mvg060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Induction of the mitochondrial nitrate-respiration (denitrification) system of the fungus Fusarium oxysporum requires the supply of low levels of oxygen (O-2). Here we show that O-2 and nitrate (NO3-) respiration function simultaneously in the mitochondria of fungal cells incubated under hypoxic, denitrifying conditions in which both O-2 and NO3- act as the terminal electron acceptors. The NO3- and nitrite (NO2-) reductases involved in fungal denitrification share the mitochondrial respiratory chain with cytochrome oxidase. F oxysporum cytochrome c(549) can serve as an electron donor for both NO2- reductase and cytochrome oxidase. We are the first to demonstrate hybrid respiration in respiring eukaryotic mitochondria.

引用

页码：461 / 465

页数：5

共 23 条

[1] Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions [J].