Loss-of-function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis

被引:136
作者
Cao, SQ [1 ]
Xu, QT [1 ]
Cao, YJ [1 ]
Qian, K [1 ]
An, K [1 ]
Zhu, Y [1 ]
Hu, BZ [1 ]
Zhao, HF [1 ]
Kuai, BK [1 ]
机构
[1] Fudan Univ, Sch Life Sci, Minist Educ, Key Lab Biodivers Sci & Ecol Engn,Dept Biochem, Shanghai 200433, Peoples R China
关键词
D O I
10.1111/j.1399-3054.2004.00432.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Brassinosteroids (BRs) play an essential role in plant growth and development, and have been implicated in many physiological responses. However, little is known about the role of BRs in the plant response to oxidative stress. In this study, we identified a novel insertion allele (det2-9) of the DET2 gene in Arabidopsis based on molecular, physiological and genetic approaches. We found that the det2 mutant exhibited an enhanced resistance to oxidative stress. The enhanced oxidative stress resistance in det2 plants was correlated with a constitutive increase in superoxide dismutase (SOD) activity and increased transcript levels of the defence gene catalase (CAT). To our knowledge, these results demonstrate, for the first time, that loss-of-function mutations in the DET2 gene lead to an enhanced oxidative stress resistance in Arabidopsis. A general explanation is that the long-term BR deficiency in the det2 mutant results in a constant in vivo physiological stress that, in turn, activates the constitutive expression of some defence genes and, consequently, the activities of related enzymes.
引用
收藏
页码:57 / 66
页数:10
相关论文
共 52 条
[1]  
Beauchamp C., 1971, ANAL BIOCHEM, V44, P276, DOI DOI 10.1016/0003-2697(71)90370-8
[2]   ASSIGNMENT OF 30 MICROSATELLITE LOCI TO THE LINKAGE MAP OF ARABIDOPSIS [J].
BELL, CJ ;
ECKER, JR .
GENOMICS, 1994, 19 (01) :137-144
[3]   Brassinosteroids and plant steroid hormone signaling [J].
Bishop, GJ ;
Koncz, C .
PLANT CELL, 2002, 14 :S97-S110
[4]   Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings [J].
Borsani, O ;
Valpuesta, V ;
Botella, MA .
PLANT PHYSIOLOGY, 2001, 126 (03) :1024-1030
[5]   THE INFLUENCE OF BRASSINOSTEROID ON GROWTH AND PARAMETERS OF PHOTOSYNTHESIS OF WHEAT AND MUSTARD PLANTS [J].
BRAUN, P ;
WILD, A .
JOURNAL OF PLANT PHYSIOLOGY, 1984, 116 (03) :189-196
[6]  
CHORY J, 1991, PLANT CELL, V3, P445, DOI 10.1105/tpc.3.5.445
[7]   Brassinosteroids: Essential regulators of plant growth and development [J].
Clouse, SD ;
Sasse, JM .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :427-451
[8]  
Devi SR, 1998, PLANT SCI, V138, P157, DOI 10.1016/S0168-9452(98)00161-7
[9]   Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings [J].
Dhaubhadel, S ;
Chaudhary, S ;
Dobinson, KF ;
Krishna, P .
PLANT MOLECULAR BIOLOGY, 1999, 40 (02) :333-342
[10]   LEAF SENESCENCE - CORRELATED WITH INCREASED LEVELS OF MEMBRANE-PERMEABILITY AND LIPID-PEROXIDATION, AND DECREASED LEVELS OF SUPEROXIDE-DISMUTASE AND CATALASE [J].
DHINDSA, RS ;
PLUMBDHINDSA, P ;
THORPE, TA .
JOURNAL OF EXPERIMENTAL BOTANY, 1981, 32 (126) :93-101