Isolation and characterization of an Azotobacter vinelandii algK mutant

被引：34

作者：

Mejía-Ruíz, H ^{[1
]}

Moreno, S ^{[1
]}

Guzmán, J ^{[1
]}

Nájera, R ^{[1
]}

León, R ^{[1
]}

Soberón-Chávez, G ^{[1
]}

Espín, G ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Mol Microbiol, Cuernavaca 62250, Morelos, Mexico

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 156卷 / 01期

关键词：

alginate; algK; transcription analysis;

D O I：

10.1016/S0378-1097(97)00409-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Random Tn5 mutagenesis over Azotobacter vinelandii mucoid strain ATCC 9046 produced strain LA21, a non-mucoid, non-encysting mutant, carrying the Tn5 insertion within a gene homologous to algK from Pseudomonas aeruginosa encoding a periplasmic protein. algK, algJ and algG were shown to be transcribed as part of the palg8-alg44-algK-algJ-algG operon. A non-polar algK mutant was constructed and showed a non-mucoid phenotype, indicating that algK is essential for alginate production.

引用

页码：101 / 106

页数：6

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