Two major Spanish clones of penicillin-resistant Streptococcus pneumoniae in Portuguese isolates of clinical origin

被引：6

作者：

Caniça, M ^{[1
]}

Dias, R ^{[1
]}

Vaz-Pato, MV ^{[1
]}

Carvalho, C ^{[1
]}

机构：

[1] Natl Inst Hlth Dr Ricardo Jorge, Antibiot Resistance Unit, Ctr Bacteriol, P-1649016 Lisbon, Portugal

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2003年 / 51卷 / 02期

关键词：

Streptococcus pneumoniae; Portugal; drug-resistant clones; genetic relatedness;

D O I：

10.1093/jac/dkg078

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We studied the genetic relatedness of 47 Portuguese penicillin-resistant 9V and 23F Streptococcus pneumoniae of clinical origin, using pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism analysis. PFGE fingerprinting showed that 24 isolates of serotype 9V and 23 isolates of serotype 23F were variants of the Spain(9V)-3 and Spain(23F)-1 clones, respectively. Fingerprinting of pbp1a, pbp2b and pbp2x genes showed that all the penicillin-resistant clones gave similar HinfI restriction patterns. In this study, serotypes 9V and 23F have different clonal origins and identical PBP genotypes, suggesting a horizontal transfer of resistance. Visual and computer-assisted analysis of PFGE patterns correlated well (r = 0.983).

引用

页码：409 / 414

页数：6

共 10 条

[1]

Carvalho C, 1996, PATHOL BIOL, V44, P430

[2]

CARVALHO C, 1999, M MICR 99 LUS PORT 1, P185

[3] HORIZONTAL TRANSFER OF MULTIPLE PENICILLIN-BINDING PROTEIN GENES, AND CAPSULAR BIOSYNTHETIC GENES, IN NATURAL-POPULATIONS OF STREPTOCOCCUS-PNEUMONIAE [J].