Using GFP-ligand fusions to measure receptor-mediated endocytosis in living cells

被引:11
作者
Medina-Kauwe, LK [1 ]
Chen, XH [1 ]
机构
[1] Univ So Calif, Dept Biochem, Inst Med Genet, Keck Sch Med, Los Angeles, CA 90033 USA
来源
VITAMINS AND HORMONES - ADVANCES IN RESEARCH AND APPLICATIONS, VOL 65 | 2002年 / 65卷
关键词
D O I
10.1016/S0083-6729(02)65060-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recombinant DNA technology has enabled the production of many types of chimeric proteins containing heterologous functional domains that have served a variety of useful capacities for cell biology research. Among proteins gaining wide use as a fusion partner is Aequorea victoria green fluorescent protein (GFP). GFP has been employed by numerous groups as a reporter gene for cell transfection and as an autofluorescent tag by recombinant fusion to foreign sequences. Here we describe the use of GFP as a tag for ligands, and provide examples of how purified recombinant GFP-ligand fusion proteins may be used to detect ligand-receptor interactions, including receptor-mediated endocytosis. Both its utility and limitations are discussed. © 2002.
引用
收藏
页码:81 / 95
页数:15
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