Fungal attack on coal II. Solubilization of low-rank coal by filamentous fungi

被引:41
作者
Hofrichter, M [1 ]
Bublitz, F [1 ]
Fritsche, W [1 ]
机构
[1] Univ Jena, Inst Microbiol, D-07743 Jena, Germany
关键词
coal solubilization; lignite; humic acids; fungi; deuteromycetes; molds; basidiomycetes; oxidases; peroxidases; ABTS;
D O I
10.1016/S0378-3820(97)00015-5
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The solubilization of low-rank coal by fungi is not accompanied by its depolymerization, which was proven by several screening programs, A total number of 728 fungal strains were yet tested for solubilizing a German low-rank coal (lignite). The coal were used oxidatively pretreated (3% H2O2) and natively. During the first screening, the nitrogen content of the media were reduced to select fungi, which solubilize coal under nitrogen-limited conditions. Among 480 micromycetous fungi tested, at least ten strains were able to solubilize native low-rank coal pieces on a nitrogen-reduced agar-medium. They all belong to the conidiospores forming deuteromycetes (molds). Under 'solubilizing' conditions, only two of these ten strains showed weak extracellular activities (oxidases, peroxidases) toward 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate) [ABTS]. In a second screening, 256 wood and litter decaying basidiomycetes, above all white-rot fungi, were tested for their ability to solubilize low-rank coal under ligninolytic conditions; not any strain was able to form black coal droplets or conspicuous diffusion areas. By using a nitrogen-rich medium, a few basidiomycetes showed also coal solubilizing activities, however, their ligninolytic enzymes were inhibited. The 'typical' solubilization of low-rank coal (formation of black liquids from solid coal particles) by molds depends mainly on the nitrogen content of the medium (resulting in higher pH values) as well as on the oxidation grade of coal (e.g. H2O2-pretreatment). Extracellular oxidases and peroxidases, both ligninolytic and nonligninolytic ones, seem to play a minor, (or not any), role for this process. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:55 / 64
页数:10
相关论文
共 34 条
[1]   STUDIES ON THE MICROBIAL-DEGRADATION OF A NIGERIAN PREOXIDIZED SUBBITUMINOUS COAL [J].
ACHI, OK .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 1993, 31 (04) :293-303
[2]   PHENOL OXIDASE ACTIVITY AND PIGMENT SYNTHESIS IN CONIDIOSPORES OF PENICILLIUM-CYCLOPIUM [J].
BARTSCH, E ;
LERBS, W ;
LUCKNER, M .
ZEITSCHRIFT FUR ALLGEMEINE MIKROBIOLOGIE, 1979, 19 (02) :75-82
[3]   CELL-FREE SOLUBILIZATION OF COAL BY POLYPORUS-VERSICOLOR [J].
COHEN, MS ;
BOWERS, WC ;
ARONSON, H ;
GRAY, ET .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (12) :2840-2843
[4]   DEGRADATION OF COAL BY THE FUNGI POLYPORUS-VERSICOLOR AND PORIA-MONTICOLA [J].
COHEN, MS ;
GABRIELE, PD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 44 (01) :23-27
[5]  
CRAWFORD DL, 1993, MICROBIAL TRANSFORMA
[6]   BIOLOGICAL COAL CONVERSIONS [J].
FAISON, BD .
CRITICAL REVIEWS IN BIOTECHNOLOGY, 1991, 11 (04) :347-366
[7]   PRODUCTION OF COAL-SOLUBILIZING ACTIVITY BY PAECILOMYCES SP DURING SUBMERGED GROWTH IN DEFINED LIQUID-MEDIA [J].
FAISON, BD ;
LEWIS, SN .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1989, 20-1 :743-752
[8]  
FAKOUSSA R, 1992, BIOENGIN, V8, P21
[9]  
FAKOUSSA RM, 1990, P 1 INT S BIOL PROC, V4, P3
[10]  
FAKOUSSA RM, 1981, THESIS F WILHELMS U