Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules

被引：4

作者：

Cheng, PC ^{[1
]}

Lin, BL ^{[1
]}

Kao, FJ ^{[1
]}

Sun, CK ^{[1
]}

Wang, YS ^{[1
]}

Liu, TM ^{[1
]}

Wan, YM ^{[1
]}

Chen, JC ^{[1
]}

Huang, MK ^{[1
]}

Johnson, I ^{[1
]}

机构：

[1] Acad Sinica, Inst Mol Biol, Taipei 115, Taiwan

来源：

OPTICAL SENSING, IMAGING, AND MANIPULATION FOR BIOLOGICAL AND BIOMEDICAL APPLICATIONS | 2000年 / 4082卷

关键词：

multi-photon fluorescence spectroscopy; two-photon; three-photon; fluorescence probes; biological probes; auto-fluorescence; bio-chip;

D O I：

10.1117/12.390527

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the groper selection of spectral setting/ dichroic beam splitter in the setup of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio -chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

引用

页码：87 / 91

页数：5