Optimal 96-well plate set-up to avoid ethanol volatility when assessing ethanol cytotoxicity

被引：19

作者：

Mitchell, JJ ^{[1
]}

Paiva, M ^{[1
]}

Heaton, MB ^{[1
]}

机构：

[1] Univ Florida, Coll Med, Dept Neurosci, Hlth Sci Ctr, Gainesville, FL 32610 USA

来源：

ALCOHOL | 1998年 / 15卷 / 02期

关键词：

ethanol cytotoxicity; MTT; ethanol volatility; 96-well culture plates;

D O I：

10.1016/S0741-8329(97)00124-9

中图分类号：

R194 [卫生标准、卫生检查、医药管理];

学科分类号：

摘要：

An optimal design has been established that maintains a constant level of ethanol when utilizing specific wells within a 96-well culture plate. A control (non-ethanol-containing) column is also included within each plate for direct comparisons of the cytotoxic effects of a given concentration of ethanol. (C) 1998 Elsevier Science Inc.

引用

页码：137 / 139

页数：3

共 8 条

[1] CROSS CONTAMINATION ASSOCIATED WITH THE USE OF MULTIWELL CULTURE PLATES FOR CYTOTOXICITY ASSESSMENT OF VOLATILE CHEMICALS [J].

BLEIN, O ;

RONOT, X ;

ADOLPHE, M .

CYTOTECHNOLOGY, 1991, 6 (01) :79-82

[2] EFFECTIVE STABILIZATION OF ETHANOL LEVELS IN MULTIPLE-WELL TISSUE-CULTURE PLATES [J].

BORGS, P ;

WAY, DL ;

WITTE, MH ;

WITTE, CL .

ALCOHOL, 1993, 10 (01) :31-35

[3]

BORGS P, 1991, CLIN RES, V39, pA426

[4] RAPID COLORIMETRIC ASSAY FOR CELL-GROWTH AND SURVIVAL - MODIFICATIONS TO THE TETRAZOLIUM DYE PROCEDURE GIVING IMPROVED SENSITIVITY AND RELIABILITY [J].

DENIZOT, F ;

LANG, R .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 89 (02) :271-277

[5]

FRESHNEY R, 1983, CULTURES ANIMAL CELL, P63

[6]

HURLEY MM, 1987, P SOC EXP BIOL MED, V186, P139

[7] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS [J].

MOSMANN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) :55-63

[8] STUDIES ON SUCCINATE-TETRAZOLIUM REDUCTASE SYSTEMS .3. POINTS OF COUPLING OF 4 DIFFERENT TETRAZOLIUM SALTS [J].

SLATER, TF ;

SAWYER, B ;

STRAULI, U .

BIOCHIMICA ET BIOPHYSICA ACTA, 1963, 77 (03) :383-&

← 1 →