Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody

被引：395

作者：

Dai, Lianpan ^{[1
]}

Song, Jian ^{[2
,3
]}

Lu, Xishan ^{[4
]}

Deng, Yong-Qiang ^{[5
]}

Musyoki, Abednego Moki ^{[2
,3
]}

Cheng, Huijun ^{[1
]}

Zhang, Yanfang ^{[4
]}

Yuan, Yuan ^{[2
,3
]}

Song, Hao ^{[2
,3
]}

Haywood, Joel ^{[2
,3
]}

Xiao, Haixia ^{[4
]}

Yan, Jinghua ^{[2
,3
,6
,7
,8
]}

Shi, Yi ^{[1
,2
,3
,6
,7
]}

Qin, Cheng-Feng ^{[5
]}

Qi, Jianxun ^{[2
]}

Gao, George F. ^{[1
,2
,3
,4
,6
,7
,9
,10
]}

机构：

[1] Chinese Acad Sci, Beijing Inst Life Sci, Res Network Immun & Hlth RNIH, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100071, Peoples R China

[3] Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China

[4] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China

[5] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100101, Peoples R China

[6] Shenzhen Third Peoples Hosp, Shenzhen Key Lab Pathogen & Immun, Shenzhen 518112, Peoples R China

[7] Chinese Acad Sci, Ctr Influenza Res & Early Warning CASCIRE, Beijing 100101, Peoples R China

[8] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Engn, Beijing 100101, Peoples R China

[9] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310003, Zhejiang, Peoples R China

[10] Chinese Ctr Dis Control & Prevent China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China

来源：

CELL HOST & MICROBE | 2016年 / 19卷 / 05期

基金：

中国国家自然科学基金;

关键词：

WEST-NILE-VIRUS; CROSS-REACTIVE ANTIBODIES; HUMAN MONOCLONAL-ANTIBODIES; DENGUE VIRUS; FUSION-LOOP; NEUTRALIZING ANTIBODIES; EBOLA-VIRUS; ENCEPHALITIS-VIRUS; CRYSTAL-STRUCTURE; ASIAN LINEAGE;

D O I：

10.1016/j.chom.2016.04.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Zika virus (ZIKV), a mosquito-borne flavivirus, is a current global public health concern. The flavivirus envelope (E) glycoprotein is responsible for virus entry and represents a major target of neutralizing antibodies for other flaviviruses. Here, we report the structures of ZIKV E protein at 2.0 angstrom and in complex with a flavivirus broadly neutralizing murine antibody 2A10G6 at 3.0 angstrom. ZIKV-E resembles all the known flavivirus E structures but contains a unique, positively charged patch adjacent to the fusion loop region of the juxtaposed monomer, which may influence host attachment. The ZIKV-E-2A10G6 complex structure reveals antibody recognition of a highly conserved fusion loop. 2A10G6 binds to ZIKV-E with high affinity in vitro and neutralizes currently circulating ZIKV strains in vitro and in mice. The E protein fusion loop epitope represents a potential candidate for therapeutic antibodies against ZIKV.

引用

页码：696 / 704

页数：9

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