Nuclear translocation of the transcription factor STAT5 in the rat brain after systemic leptin administration

被引：59

作者：

Muetze, Joerg ^{[1
]}

Roth, Joachim ^{[1
]}

Gerstberger, Ruediger ^{[1
]}

Huebschle, Thomas ^{[1
]}

机构：

[1] Univ Giessen, Dept Vet Physiol, D-35392 Giessen, Germany

来源：

NEUROSCIENCE LETTERS | 2007年 / 417卷 / 03期

关键词：

leptin-target cells; transcription factors; signal transducer and activator of transcription 5 (STAT5); hypothalamus;

D O I：

10.1016/j.neulet.2007.02.074

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Leptin binding to its functional receptor stimulates JAK-STAT-signaling pathway, which finally results in activation and nuclear translocation of transcription factors of the signal transducer and activator of transcription (STAT) family, namely of STAT3. Here we report for the first time that systemic treatment with leptin (5 mg/kg; intraperitoneal injection) also increased the number of nuclear STAT5 signals in the hypothalamus. In particular, the entire arcuate nucleus (ARC), the ventral premammilary nucleus (PMV), and the supraoptic nucleus (SO) showed an enhanced nuclear STAT5 translocation in response to leptin when compared to saline, 120 min after the respective injection. Co-localization studies revealed that a high percentage of those STAT5-responsive cells proved to be neurons. In addition, some astrocytes within the ARC showed nuclear STAT5 signals. The functional relevance of leptin-induced nuclear STAT5 activation in hypothalamic cells still has to be determined. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：286 / 291

页数：6

共 21 条

[1] Interaction between leptin and insulin signaling pathways differentially affects JAK-STAT and PI 3-kinase-mediated signaling in rat liver [J].

Carvalheira, JBC ;

Ribeiro, EB ;

Folli, F ;

Velloso, LA ;

Saad, MJA .

BIOLOGICAL CHEMISTRY, 2003, 384 (01) :151-159

[2]

Elmquist JK, 1998, J COMP NEUROL, V395, P535

[3] Anatomic localization of alternatively spliced leptin receptors (Ob-R) in mouse brain and other tissues [J].

Fei, H ;

Okano, HJ ;

Li, C ;

Lee, GH ;

Zhao, C ;

Darnell, R ;

Friedman, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (13) :7001-7005

[4] Leptin and the regulation of body weight in mammals [J].

Friedman, JM ;

Halaas, JL .

NATURE, 1998, 395 (6704) :763-770

[5]

Håkansson ML, 1998, J NEUROSCI, V18, P559

[6] Brain stem is a direct target for leptin's action in the central nervous system [J].

Hosoi, T ;

Kawagishi, T ;

Okuma, Y ;

Tanaka, J ;

Nomura, Y .

ENDOCRINOLOGY, 2002, 143 (09) :3498-3504

[7] Leptin-induced nuclear translocation of STAT3 immunoreactivity in hypothalamic nuclei involved in body weight regulation [J].

Hübschle, T ;

Thom, E ;

Watson, A ;

Roth, J ;

Klaus, S ;

Meyerhof, W .

JOURNAL OF NEUROSCIENCE, 2001, 21 (07) :2413-2424

[8] Leptin induces cyclooxygenase-2 via an interaction with interleukin-1β in the rat brain [J].

Inoue, Wataru ;

Poole, Stephan ;

Bristow, Adrian F. ;

Luheshi, Giamal N. .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2006, 24 (08) :2233-2245

[9] Nuclear translocation of STAT5 and increased expression of Fos related antigens (FRAs) in hypothalamic dopaminergic neurons after prolactin administration [J].

Lerant, A ;

Kanyicska, B ;

Freeman, ME .

BRAIN RESEARCH, 2001, 904 (02) :259-269

[10] Leptin signaling in the hypothalamus of normal rats in vivo [J].

McCowen, KC ;

Chow, JC ;

Smith, RJ .

ENDOCRINOLOGY, 1998, 139 (11) :4442-4447

← 1 2 3 →