Progenitor Cell Expansion and Organ Size of Mouse Adrenal Is Regulated by Sonic Hedgehog

被引:89
作者
Huang, Chen-Che Jeff [1 ]
Miyagawa, Shinichi [2 ]
Matsumaru, Daisuke [2 ]
Parker, Keith L. [3 ]
Yao, Humphrey Hung-Chang [1 ]
机构
[1] Univ Illinois, Dept Vet Biosci, Urbana, IL 61802 USA
[2] Kumamoto Univ, Ctr Anim Resources & Dev, Kumamoto 8600811, Japan
[3] Univ Texas SW Med Ctr Dallas, Dept Internal Med, Dallas, TX 75390 USA
关键词
TARGETED DISRUPTION; HYPOPLASIA CONGENITA; STEM-CELLS; CORTEX; MICE; RECEPTOR; GENE; DIFFERENTIATION; EXPRESSION; DAX-1;
D O I
10.1210/en.2009-0814
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The adrenal capsule is postulated to harbor stem/progenitor cells, the progenies of which contribute to the growth of adrenocortex. We discovered that cells in the adrenal capsule are positive for Ptch1 and Gli1, genes indicative of responsiveness to the stimulation of Hedgehog (Hh) ligands. On the other hand, Sonic hedgehog (Shh), one of the mammalian Hh ligands, is expressed in the adrenocortex underneath the adrenal capsule, possibly acting upon the Hh-Responsive capsule. To investigate the functional significance of Shh in adrenal growth, we ablated Shh in an adreno-cortex-specific manner using the Steroidogenic factor 1-Cre mouse. Loss of Shh in the adrenocortex led to reduced proliferation of capsular cells and a 50-75% reduction in adrenocortex thickness and adrenal size. The remaining adrenocortex underwent proper zonation and was able to synthesize steroids, indicating that Shh is dispensable for differentiation of adrenocortex. When these animals reached adulthood, their adrenocortex did not undergo compensatory growth in response to a high level of plasma ACTH, and the size of the adrenal remained significantly smaller than the control adrenal. Using a genetic lineage-tracing model, we further demonstrated that the Hh-responding cells in the adrenal capsule migrated centripetally into the adrenocortex. Our results not only provide the genetic evidence to support that the adrenal capsule contributes to the growth of adrenocortex in both fetal and adult life but also identify a novel role of Shh in this process. ( Endocrinology 151: 1119-1128, 2010)
引用
收藏
页码:1119 / 1128
页数:10
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