Expression and regulation of vascular endothelial growth factor in human pulmonary epithelial cells

被引:120
作者
Boussat, S
Eddahibi, S
Coste, A
Fataccioli, V
Gouge, M
Housset, B
Adnot, S
Maitre, B
机构
[1] Univ Paris 12, Assistance Publ Hop Paris, Hop Henri Mondor & Intercommunal Creteil, INSERM,U492, F-94010 Creteil, France
[2] Univ Paris 12, Assistance Publ Hop Paris, Hop Henri Mondor & Intercommunal Creteil, Dept Physiol, F-94010 Creteil, France
[3] Univ Paris 12, Assistance Publ Hop Paris, Hop Henri Mondor & Intercommunal Creteil, Serv Pneumol, F-94010 Creteil, France
[4] Univ Paris 12, Assistance Publ Hop Paris, Hop Henri Mondor & Intercommunal Creteil, Serv Otorhinolaryngol, F-94010 Creteil, France
关键词
angiogenesis; bronchial epithelial cells; lung injury;
D O I
10.1152/ajplung.2000.279.2.L371
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Vascular endothelial growth factor (VEGF) is a potent endothelial cell growth and permeability factor highly expressed in rodent alveolar epithelium after injury and repair. To investigate VEGF synthesis in human lung epithelial cells, we examined VEGF expression by cultured cells under basal conditions and after cytokine treatment or oxidative stress. Basal VEGF expression was detected in transformed human epithelial cell lines (A549 and 1HAEo(-)) and in primary human bronchial epithelial cells with RT-PCR, Western blot, and immunocytochemistry. Among the cytokines tested, only transforming growth factor-beta 1 increased the levels of excreted VEGF(165) as measured by ELISA. Under hypoxia (0% O-2 for 24 h), the VEGF(165) level increased fivefold, and this effect was O-2 concentration dependent. VEGF concentrations in the medium of all the cell types studied reached values similar to those found in bronchoalveolar lavage fluids from normal patients. Endothelial cells (human umbilical vein endothelial cells) exposed to conditioned medium from primary bronchial epithelial cell cultures showed an increased growth rate, which was inhibited in the presence of a specific neutralizing antibody to VEGF. These results suggest that lung epithelial cells participate in the endothelial repair and angiogenesis that follow lung injury through the synthesis of VEGF.
引用
收藏
页码:L371 / L378
页数:8
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