Cloning of a type I cytokine receptor most related to the IL-2 receptor β chain

We have identified a type I cytokine receptor, which we have termed novel interleukin receptor(NILR), that is most related to the IL-2 receptor beta chain (IL-2R beta) and physically adjacent to the IL-4 receptor ru chain gene on chromosome 16, NILR mRNA is most highly expressed in thymus and spleen, and is induced by phyto-hemagglutinin in human peripheral blood mononuclear cells. NILR protein was detected on human 7 cell lymphotropic virus type I-transformed T cell lines, Raji B cells, and YT natural killer-like cells. Artificial homodimerization of the NILR cytoplasmic domain confers proliferation to Ba/F3 murine pro-B cells but not to 32D myeloid progenitor cells or CTLL-2 murine helper T cells. In these latter cells, heterodimerization of IL-2R beta and the common cytokine receptor gamma chain (gamma(c)) cytoplasmic domains allows potent proliferation, whereas such heterodimerization of NILR with ye does not. This finding suggests that NILR has signaling potential but that a full understanding of its signaling partner(s) is not yet clear. Like IL-2R beta, NILR associates with Jak1 and mediates stats activation.