Screening of DNA aptamer which binds to α-synuclein

被引：44

作者：

Tsukakoshi, Kaori ^{[1
]}

Harada, Ryuichi ^{[1
]}

Sode, Koji ^{[1
]}

Ikebukuro, Kazunori ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 05期

关键词：

alpha-Synuclein; Alzheimer's disease; DNA aptamer; Lewy bodies; Parkinson's disease; PRION-PROTEIN; ALZHEIMERS-DISEASE; ENZYME SUBUNIT; RNA APTAMERS; SELECTION; SEQUENCE; LIGANDS;

D O I：

10.1007/s10529-010-0200-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

alpha-Synuclein is a native, unfolded protein that causes several neurodegenerative diseases such as dementia with Lewy bodies and Parkinson's disease. We have now identified the first DNA aptamers against alpha-synuclein using native PAGE applied to the SELEX method. We call this aptamer "M5-15"; it is the alpha-synuclein-bound aptamer and was isolated after four cycles of screening. M5-15 is composed of three stem-loop structures that may play an important role in the binding to alpha-synuclein. Moreover, M5-15 specifically binds to the alpha-synuclein monomer and oligomer. We expect that this aptamer will become a useful tool in alpha-synuclein analysis and diagnosis.

引用

页码：643 / 648

页数：6

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