Stress proteins (hsp70, hsp60) induced in isopods and nematodes by field exposure to metals in a gradient near Avonmouth, UK

被引:64
作者
Arts, MJSJ
Schill, RO
Knigge, T
Eckwert, H
Kammenga, JE
Köhler, HR
机构
[1] Univ Wageningen & Res Ctr, Nematol Lab, NL-6709 PD Wageningen, Netherlands
[2] Univ Tubingen, Inst Zool, D-72076 Tubingen, Germany
关键词
biomarker; heat shock proteins; nematode; isopods; metal polluted soil; field bioassay;
D O I
10.1007/s10646-003-4473-5
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Heat shock proteins (hsps) are potential biomarkers for monitoring environmental pollution. In this study, the use of hsps as biomarkers in field bioassays was evaluated in terrestrial invertebrates exposed to a metal gradient near Avonmouth, UK. We investigated the hsp70 response in resident and transplanted isopods of the species Oniscus asellus and Porcellio scaber and the hsp60 response in transplanted nematodes of the species Plectus acuminatus in six field sites along the metal gradient. Considerable differences were detected in the stress responses between nematodes and isopods (isopods responded in a gradientspecific manner, nematodes did not), the two isopod species and the transplanted and resident specimens of each isopod species: in the sites closest to the smelter, O. asellus residents showed high hsp70 levels while O. asellus transplanted from an unpolluted site displayed comparatively low hsp70 levels. For P. scaber, it was just the opposite. In resident isopod populations of both species, tolerant phenotypes were revealed in the most contaminated field sites. The hsp70 level in both isopod species was a suitable biomarker of effect (but of exposure only in non-tolerant individuals) even in long-term metal -contaminated field sites. The hsp60 response in the nematode alone was not a suitable biomarker for heavily contaminated soils. However, it had indicative value when related to the hsp70 response in the isopods and could be a suitable biomarker for less heavily contaminated soils.
引用
收藏
页码:739 / 755
页数:17
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共 50 条
[1]   ADDITIONAL CHARACTERISTICS OF THE POLYPHOSPHATE-ACCUMULATING ACINETOBACTER STRAIN 210A AND ITS IDENTIFICATION AS ACINETOBACTER-JOHNSONII [J].
BONTING, CFC ;
WILLEMSEN, BMF ;
AKKERMANSVANVLIET, W ;
BOUVET, PJM ;
KORTSTEE, GJJ ;
ZEHNDER, AJB .
FEMS MICROBIOLOGY ECOLOGY, 1992, 102 (01) :57-64
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   A ROLE FOR A 70-KILODATON HEAT-SHOCK PROTEIN IN LYSOSOMAL DEGRADATION OF INTRACELLULAR PROTEINS [J].
CHIANG, HL ;
TERLECKY, SR ;
PLANT, CP ;
DICE, JF .
SCIENCE, 1989, 246 (4928) :382-385
[4]  
DeBoer JM, 1996, FUND APPL NEMATOL, V19, P545
[5]   Heat-shock proteins as biomarkers of pollution [J].
dePomerai, D .
HUMAN & EXPERIMENTAL TOXICOLOGY, 1996, 15 (04) :279-285
[6]   THE TOXICITY OF ZINC TO TERRESTRIAL ISOPODS IN A STANDARD LABORATORY TEST [J].
DROBNE, D ;
HOPKIN, SP .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 1995, 31 (01) :1-6
[7]   Development of broad spectrum antibodies to heat shock protein 70s as biomarkers for detection of multiple stress by pollutants and environmental factors [J].
Dunlap, DY ;
Matsumura, F .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 1997, 37 (03) :238-244
[8]   The induction of stress proteins (hsp) in Oniscus asellus (Isopoda) as a molecular marker of multiple heavy metal exposure .1. Principles and toxicological assessment [J].
Eckwert, H ;
Alberti, G ;
Kohler, HR .
ECOTOXICOLOGY, 1997, 6 (05) :249-262
[9]  
Eisenbeis G., 1993, INFORMATIONEN NATURS, P53
[10]   MOLECULAR CHAPERONES [J].
ELLIS, RJ ;
VANDERVIES, SM .
ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :321-347