trans activity of the norovirus Camberwell proteinase and cleavage of the N-terminal protein encoded by ORF1

被引：17

作者：

Seah, EL ^{[1
]}

Marshall, JA

Wright, PJ

机构：

[1] Monash Univ, Dept Microbiol, Clayton, Vic 3800, Australia

[2] Victorian Infect Dis Reference Lab, Melbourne, Vic 3051, Australia

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 12期

关键词：

D O I：

10.1128/JVI.77.12.7150-7155.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The virus-encoded proteinase of Camberwell virus, a genogroup 2 norovirus, was synthesized in Escherichia coli. The purified proteinase had correct N and C termini and showed trans activity in cell-free assays. trans activity was also demonstrated in COS cells transfected with constructs encoding either the proteinase or a proteinase-polymerase fusion. The N-terminal protein of ORF1 was cleaved in COS cells, possibly at the site E-194/S.

引用

页码：7150 / 7155

页数：6

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