Maturation and translation mechanisms involved in the expression of a myb gene of rice

被引：30

作者：

Magaraggia, F

Solinas, G

Valle, G

Giovinazzo, G

Coraggio, I

机构：

[1] CNR, Ist Biosintesi Vegetali, I-20133 Milan, Italy

[2] Univ Padua, Dipartimento Biol, I-35121 Padua, Italy

[3] CNR, IRBA, Lecce, Italy

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 35卷 / 06期

关键词：

upstream ORF; regulated splicing; myb; stress; leucine zipper; plant;

D O I：

10.1023/A:1005821518242

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated two overlapping cDNAs coding for a MYB-related protein expressed in aerobic and anaerobic rice (Oryza sativa) roots and coleoptiles. Analysis of their sequences reveals some peculiar features, suggesting the presence of post-transcriptional regulation events: an upstream ORE two unspliced introns and a putative leucine zipper in the ORF coded by the unspliced RNA. Transient expression in protoplasts indicates that the upstream ORF inhibits expression of a downstream coding sequence. Finally, we demonstrated that anoxia, in roots, increases the ratio between the spliced and the unspliced mRNA and affects the expression of other myb-related genes.

引用

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页码：1003 / 1008

页数：6

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