Upstream promoter sequences and αCTD mediate stable DNA wrapping within the RNA polymerase-promoter open complex

被引：27

作者：

Cellai, Sara

Mangiarotti, Laura

Vannini, Nicola

Naryshkin, Nikolai

Kortkhonjia, Ekaterine

Ebright, Richard H.

Rivetti, Claudio

机构：

[1] Univ Parma, Dept Biochem & Mol Biol, I-43100 Parma, Italy

[2] Rutgers State Univ, Dept Chem, Waksman Inst, Piscataway, NJ USA

[3] Rutgers State Univ, Howard Hughes Med Inst, Piscataway, NJ USA

来源：

EMBO REPORTS | 2007年 / 8卷 / 03期

关键词：

alpha carboxy-terminal domain; atomic force microscopy; DNA wrapping; transcription; UP elements;

D O I：

10.1038/sj.embor.7400888

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that the extent of stable DNA wrapping by Escherichia coli RNA polymerase (RNAP) in the RNAP-promoter open complex depends on the sequence of the promoter and, in particular, on the sequence of the upstream region of the promoter. We further show that the extent of stable DNA wrapping depends on the presence of the RNAP alpha-subunit carboxy-terminal domain and on the presence and length of the RNAP alpha-subunit interdomain linker. Our results indicate that the extensive stable DNA wrapping observed previously in the RNAP-promoter open complex at the lambda P-R promoter is not a general feature of RNAP-promoter open complexes.

引用

收藏

页码：271 / 278

页数：8

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