Microgram-scale protein structure determination by NMR

被引：28

作者：

Aramini, James M.

Rossi, Paolo

Anklin, Clemens

Xiao, Rong

Montelione, Gaetano T. ^{[1
]}

机构：

[1] Rutgers State Univ, Dept Mol Biol & Biochem, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA

[2] Rutgers State Univ, NESG, Piscataway, NJ 08854 USA

[3] Bruker BioSpin Corp, Billerica, MA 01821 USA

[4] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Biochem & Mol Biol, Piscataway, NJ 08854 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 06期

关键词：

D O I：

10.1038/nmeth1051

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mu g (6 mu l, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.

引用

收藏

页码：491 / 493

页数：3

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