SARS coronavirus E protein forms cation-selective ion channels

被引：218

作者：

Wilson, L

Mckinlay, C

Gage, P

Ewart, G

机构：

[1] Australian Natl Univ, Biotron Ltd, Canberra, ACT 2601, Australia

[2] Australian Natl Univ, Sch Med, Canberra, ACT 2601, Australia

[3] Australian Natl Univ, John Curtin Sch Med Res, Canberra, ACT 2601, Australia

来源：

VIROLOGY | 2004年 / 330卷 / 01期

关键词：

Severe Acute Respiratory Syndrome (SARS); coronavirus; budding; E protein; ion channel; conductance; Vpu; hydrophobic; monovalent cation; mouse hepatitis virus (MHV);

D O I：

10.1016/j.virol.2004.09.033

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Severe Acute Respiratory Syndrome (SARS) is caused by a novel coronavirus (SARS-CoV). Coronaviruses including SARS-CoV encode an envelope (E) protein, a small, hydrophobic membrane protein. We report that, in planar lipid bilayers, synthetic peptides corresponding to the SARS-CoV E protein forms ion channels that are more permeable to monovalent cations than to monovalent anions. Affinity-purified polyclonal antibodies recognizing the N-terminal 19 residues of SARS-CoV E protein were used to establish the specificity of channel forination by inhibiting the ion currents generated in the presence of the E protein peptides. (C) 2004 Elsevier Inc. All rights reserved.

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页码：322 / 331

页数：10

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