SRP meets the ribosome

被引：81

作者：

Wild, K

Halic, M

Sinning, I

Beckmann, R

机构：

[1] Univ Med Sch Berlin, Charite, Inst Biochem, D-10117 Berlin, Germany

[2] Univ Heidelberg BZH, Zentrum Biochem, D-69120 Heidelberg, Germany

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2004年 / 11卷 / 11期

关键词：

D O I：

10.1038/nsmb853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cotranslational targeting directly couples synthesis of proteins to their translocation across or insertion into membranes. The signal recognition particle (SRP) and its membrane-bound receptor facilitate the targeting of the translation machinery, the ribosome, via recognition of a signal sequence in the nascent peptide chain. By combining structures of free and ribosome-bound SRP we derive a structural model describing the dynamic nature of SRP when it meets the ribosome.

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收藏

页码：1049 / 1053

页数：5

相关论文

共 35 条

[1] The ribosome regulates the GTPase of the β-subunit of the signal recognition particle receptor [J].

Bacher, G ;

Pool, M ;

Dobberstein, B .

JOURNAL OF CELL BIOLOGY, 1999, 146 (04) :723-730

[2] Regulation by the ribosome of the GTPase of the signal-recognition particle during protein targeting [J].

Bacher, G ;

Lutcke, H ;

Jungnickel, B ;

Rapoport, TA ;

Dobberstein, B .

NATURE, 1996, 381 (6579) :248-251

[3] Crystal structure of the ribonucleoprotein core of the signal recognition particle [J].

Batey, RT ;

Rambo, RP ;

Lucast, L ;

Rha, B ;

Doudna, JA .

SCIENCE, 2000, 287 (5456) :1232-+

[4] Architecture of the protein-conducting channel associated with the translating 80S ribosome [J].

Beckmann, R ;

Spahn, CMT ;

Eswar, N ;

Helmers, J ;

Penczek, PA ;

Sali, A ;

Frank, J ;

Blobel, G .

CELL, 2001, 107 (03) :361-372

[5] Mapping the signal sequence-binding site on SRP reveals a significant role for the NG domain [J].

Cleverley, RM ;

Gierasch, LM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (48) :46763-46768

[6] Role of SRP19 in assembly of the Archaeoglobus fulgidus signal recognition particle [J].

Diener, JL ;

Wilson, C .

BIOCHEMISTRY, 2000, 39 (42) :12862-12874

[7] Structural insights into the signal recognition particle [J].

Doudna, JA ;

Batey, RT .

ANNUAL REVIEW OF BIOCHEMISTRY, 2004, 73 :539-557

[8] Substrate twinning activates the signal recognition particle and its receptor [J].

Egea, PF ;

Shan, SO ;

Napetschnig, J ;

Savage, DF ;

Walter, P ;

Stroud, RM .

NATURE, 2004, 427 (6971) :215-221

[9] Signal recognition particle binds to ribosome-bound signal sequences with fluorescence-detected subnanomolar affinity that does not diminish as the nascent chain lengthens [J].

Flanagan, JJ ;

Chen, JC ;

Miao, YW ;

Shao, YL ;

Lin, JL ;

Bock, PE ;

Johnson, AE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (20) :18628-18637

[10] Heterodimeric GTPase core of the SRP targeting complex [J].

Focia, PJ ;

Shepotinovskaya, IV ;

Seidler, JA ;

Freymann, DM .

SCIENCE, 2004, 303 (5656) :373-377

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