Substitutions in the Escherichia coli RNA polymerase σ70 factor that affect recognition of extended-10 elements at promoters

被引：38

作者：

Sanderson, A ^{[1
]}

Mitchell, JE ^{[1
]}

Minchin, SD ^{[1
]}

Busby, SJW ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

FEBS LETTERS | 2003年 / 544卷 / 1-3期

基金：

英国惠康基金;

关键词：

bacterial transcription initiation; RNA polymerase; sigma(70) subunit; promoter recognition; extended-10; promoters;

D O I：

10.1016/S0014-5793(03)00500-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：199 / 205

页数：7

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