Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles

被引：36

作者：

Arakaki, A

Hideshima, S

Nakagawa, T

Niwa, D

Tanaka, T

Matsunaga, T

Osaka, T ^{[1
]}

机构：

[1] Waseda Univ, Sch Sci & Engn, Dept Appl Chem, Shinjuku Ku, Tokyo 1698555, Japan

[2] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 184, Japan

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2004年 / 88卷 / 04期

关键词：

magnetic particles; self-assembled monolayer; biotin; streptavidin; magnetic detection; biomolecular interaction;

D O I：

10.1002/bit.20262

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using magnetic nanoparticles and a silicon substrate with a self-assembled monolayer. Streptavidin was immobilized on the magnetic particles, and biotin was attached to the monolayer-modified substrate. The reaction of streptavidin-modified magnetic particles on the biotin-modified substrate was clearly observed under an optical microscope. The magnetic signals from the particles were detected using a magnetic force microscope. The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses. (C) 2004 Wiley Periodicals, Inc.

引用

页码：543 / 546

页数：4

共 13 条

[1] Enantioselective crystal growth of leucine on a self-assembled monolayer with covalently attached leucine molecules [J].