New aspects of the simultaneous analysis of amino acids and amines as their o-phthaldialdehyde derivatives by high-performance liquid chromatography -: Analysis of wine, beer and vinegar

被引：107

作者：

Kutlán, D ^{[1
]}

Molnár-Perl, I ^{[1
]}

机构：

[1] Eotvos Lorand Univ, Inst Inorgan & Analyt Chem, H-1443 Budapest 112, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 987卷 / 1-2期

关键词：

wine; beer; food analysis derivatization; LC; amino acids; amines;

D O I：

10.1016/S0021-9673(02)01538-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new high-performance liquid chromatography method is described for the simultaneous quantitation of amino acids and amines for 37 compounds (20 amino acids+17 amines), as their o-phthaldialdehyde (OPA)-3-mercaptopropionic acid derivatives, within 53 min. Based on previously documented stoichiometric and reaction mechanism studies, derivatizations have been carried out with the OPA-SH-group= 1:50 containing reagents. Reliability and reproducibility of analyses have been considerably improved. Average reproducibility data in a wide concentration range of derivatives had RSDless than or equal to3.4%. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：311 / 322

页数：12

共 9 条

[1] Behavior and characteristics of amine derivatives obtained with o-phthaldialdehyde/3-mercaptopropionic acid and with o-phthaldialdehyde/N-acetyl-L-cysteine reagents
Kutlán, D
Presits, P
Molnár-Perl, I
[J]. JOURNAL OF CHROMATOGRAPHY A, 2002, 949 (1-2) : 235 - 248
[2] Characteristics and stability of the OPA/3-mercaptopropionic acid and OPA/N-acetyl-L-cysteine derivatives of amino acids
Kutlán, D
Molnár-Perl, I
[J]. CHROMATOGRAPHIA, 2001, 53 (Suppl 1) : S188 - S198
[3] KUTLAN D, UNPUB J CHROMATOGR
[4] Advances in the evaluation of the stability and characteristics of the amino acid and amine derivatives obtained with the o-phthaldialdehyde/3-mercaptopropionic acid and o-phthaldialdehyde/N-acetyl-L-cysteine reagents -: High-performance liquid chromatography-mass spectrometry study
Mengerink, Y
Kutlán, D
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Csámpai, A
Molnár-Perl, I
[J]. JOURNAL OF CHROMATOGRAPHY A, 2002, 949 (1-2) : 99 - 124
[5] Quantitation of amino acids and amines in the same matrix by high-performance liquid chromatography, either simultaneously or separately
Molnár-Perl, I
[J]. JOURNAL OF CHROMATOGRAPHY A, 2003, 987 (1-2) : 291 - 309
[6] Derivatization and chromatographic behavior of the o-phthaldialdehyde amino acid derivatives obtained with various SH-group-containing additives
Molnár-Perl, I
[J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 913 (1-2) : 283 - 302
[7] Comparison of the stability and UV and fluorescence characteristics of the o-phthaldialdehyde/3-mercaptopropionic acid and o-phthaldialdehyde/N-acetyl-L-cysteine reagents and those of their amino acid derivatives
Molnár-Perl, I
Bozor, I
[J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 798 (1-2) : 37 - 46
[8] Stability and characteristics of the o-phthaldialdehyde/3-mercaptopropionic acid and o-phthaldialdehyde/N-acetyl-L-cysteine reagents and their amino acid derivatives measured by high-performance liquid chromatography
Molnár-Perl, I
Vasanits, A
[J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 835 (1-2) : 73 - 91
[9] Retention/quantitation properties of the o-phthaldialdehyde-3-mercaptopropionic acid and the o-phthaldialdehyde-N-acetyl-L-cysteine amino acid derivatives in reversed-phase high-performance liquid chromatography
Vasanits, A
Kutlán, D
Sass, P
Molnár-Perl, I
[J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 870 (1-2) : 271 - 287

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