Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies

被引：42

作者：

Diogo, MM

Ribeiro, S

Queiroz, JA

Monteiro, GA

Perrin, P

Tordo, N

Prazeres, DMF ^{[1
]}

机构：

[1] Inst Super Tecn, Ctr Engn Biol & Quim, P-1049001 Lisbon, Portugal

[2] Univ Beira Interior, Dept Quim, P-6200 Covilha, Portugal

[3] Inst Pasteur, Lab Lyssavirus, Paris, France

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 17期

关键词：

DNA vaccines; hydrophobic interaction chromatography; plasmid DNA; rabies;

D O I：

10.1023/A:1005665003375

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The purification of a DNA vaccine against rabies by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatised with 1,4-butanediol diglycidyl ether was scaled up 60 times. The purification profile was not affected by increased loadings (up to 15 mg DNA) and a product with a consistent quality was obtained. Fourteen mg of plasmid with an HPLC purity of 100% were obtained in one run, corresponding to a 95% yield.

引用

页码：1397 / 1400

页数：4

共 10 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] PROTEIN MEASUREMENT USING BICINCHONINIC ACID - ELIMINATION OF INTERFERING SUBSTANCES [J].