Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine

被引：31

作者：

Wu, Xing Rong

Kenzior, Alexander

Willmot, David

Scanlon, Sarah

Chen, Zhihong

Topin, Andrey

He, Shao Hua

Acevedo, Ashley

Folk, William R.

机构：

[1] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA

[2] Univ Missouri, Plant Sci Unit, Columbia, MO 65211 USA

[3] Agilent Technol, Wilmington, DE 19808 USA

[4] Nichols Inst Diagnost, San Clemente, CA 92673 USA

[5] Sequoia Pharmaceut Inc, Gaithersburg, MD 20879 USA

[6] Univ Missouri, Analyt Serv, Columbia, MO USA

来源：

PLANT JOURNAL | 2007年 / 50卷 / 04期

关键词：

lysine; tRNA; aminoacyl tRNA synthetase; translational reading; zeins;

D O I：

10.1111/j.1365-313X.2007.03076.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The Arabidopsis thaliana lysyl tRNA synthetase (AtKRS) structurally and functionally resembles the well-characterized prokaryotic class IIb KRS, including the propensity to aminoacylate tRNA(Lys) with suboptimal identity elements, as well as non-cognate tRNAs. Transient expression of AtKRS in carrot cells promotes aminoacylation of such tRNAs in vivo and translational recoding of lysine at nonsense codons. Stable expression of AtKRS in Zea mays causes translational recoding of lysine into zeins, significantly enriching the lysine content of grain.

引用

页码：627 / 636

页数：10

共 72 条

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