Porphyrin - Apidaecin Conjugate as a New Broad Spectrum Antibacterial Agent

被引：57

作者：

Dosselli, Ryan ^{[1
]}

Gobbo, Marina ^{[2
]}

Bolognini, Erika ^{[2
]}

Campestrini, Sandro ^{[2
]}

Reddi, Elena ^{[1
]}

机构：

[1] Univ Padua, Dept Biol, I-35131 Padua, Italy

[2] Univ Padua, Dept Chem Sci, I-35131 Padua, Italy

来源：

ACS MEDICINAL CHEMISTRY LETTERS | 2010年 / 1卷 / 01期

关键词：

Antimicrobial PDT; antimicrobial peptides; porphyrin; apidaecin; photosensitizer-peptide conjugate; PEPTIDES;

D O I：

10.1021/ml900021y

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The conjugation of the cationic antimicrobial peptide, apidaecin Ib, to the anionic photosensitizer, 5(4'-carboxyphenyl)-10,15,20-triphenylporphyrin (cTPP), afforded a new antibacterial agent effective, under light activation, against both Gram-positive and Gram-negative bacteria At low concentrations (15-15 mu M) the conjugate was able to reduce the survival of Escherichia coli cells by 3-4 log(10), and most notably, it resulted photoactive also against hard-to-treat Pseudomonas aeruginosa. although at higher concentration (60 mu M) Under similar conditions, the photosensitizer alone was only photoactive against Staphylococcus aureus while the unconjugated peptide was inactive against all the bacterial strains tested This study shows the possibility of obtaining new broad-spectrum apidaecin photosensitizer conjugates with potent antibacterial activity

引用

页码：35 / 38

页数：4

共 18 条

[1]

[Anonymous], 2006, METH DIL ANT SUSC TE, Vseventh

[2]

CASTEELS P, 1994, BIOCHEM BIOPH RES CO, V199, P1551

[3] Lethal effects of apidaecin on Escherichia coli involve sequential molecular interactions with diverse targets [J].