Optimization of cellulase-free xylanase activity produced by Bacillus coagulans BL69 in solid-state cultivation

被引：61

作者：

Heck, JX ^{[1
]}

Flôres, SH ^{[1
]}

Hertz, PF ^{[1
]}

Ayub, MAZ ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Inst Food Sci & Technol, BR-91501970 Porto Alegre, RS, Brazil

来源：

PROCESS BIOCHEMISTRY | 2005年 / 40卷 / 01期

关键词：

cellulase-free xylanase; solid-state cultivation; bioprocess optimization; central composite design; Bacillus coagulans;

D O I：

10.1016/j.procbio.2003.11.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 22 factorial design was performed to find the best pH and temperature for xylonolytic activity of cellulase-free Bacillus coagulans isolated from the Amazon environment. Solid-state cultivation was carried out on an inexpensive and abundant agro-industrial soybean residue. The central composite design (CCD) used for the analysis of treatment combinations showed that a second order polynomial regression model was in good agreement with experimental results, with R-2 = 0.8319 (P < 0.05). The maximum activity was obtained over a large range of temperatures (45-75 degreesC) and pH (4.5-10.0). Enzymic activity was maintained in heated extracts up to 50 degreesC, suggesting that the xylanases of B. coagulans are thermo-tolerant biocatalysts, being of interests for biobleaching processes. The crude enzyme extract hydrolyzed kraft pulp cellulose and its activity was stimulated by Co2+, Mn2+ and beta-mercaptoethanol but inhibited by Fe3+, Cu2+, Ca2+, Zn2+, Ba2+, Mg2+ and by EDTA. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：107 / 112

页数：6

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