Normalisation of cereal endosperm EST libraries for structural and functional genomic analysis

被引：19

作者：

Ali, S ^{[1
]}

Holloway, B ^{[1
]}

Taylor, WC ^{[1
]}

机构：

[1] CSIRO, Div Plant Ind, Canberra, ACT 2601, Australia

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2000年 / 18卷 / 02期

关键词：

barley; endosperm; EST library; normalisation;

D O I：

10.1007/BF02824020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method for the normalisation of cDNA libraries is presented. The method has been used to normalise barley and wheat endosperm libraries. Gene probes for transcripts present at different frequencies in the primary library were used to evaluate the effectiveness of the normalisation process.

引用

收藏

页码：123 / 132

页数：10

相关论文

共 11 条

[1] Normalization and subtraction: Two approaches to facilitate gene discovery [J].

Bonaldo, MDF ;

Lennon, G ;

Soares, MB .

GENOME RESEARCH, 1996, 6 (09) :791-806

[2] Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].

Ewing, B ;

Green, P .

GENOME RESEARCH, 1998, 8 (03) :186-194

[3] Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment [J].

Ewing, B ;

Hillier, L ;

Wendl, MC ;

Green, P .

GENOME RESEARCH, 1998, 8 (03) :175-185

[4] SEQUENCE COMPLEXITY OF NUCLEAR AND POLYSOMAL RNA IN LEAVES OF TOBACCO PLANT [J].

GOLDBERG, RB ;

HOSCHEK, G ;

KAMALAY, JC ;

TIMBERLAKE, WE .

CELL, 1978, 14 (01) :123-131

[5] AN EQUALIZED CDNA LIBRARY BY THE REASSOCIATION OF SHORT DOUBLE-STRANDED CDNAS [J].

KO, MSH .

NUCLEIC ACIDS RESEARCH, 1990, 18 (19) :5705-5711

[6] CONSTRUCTION OF A UNIFORM-ABUNDANCE (NORMALIZED) CDNA LIBRARY [J].

PATANJALI, SR ;

PARIMOO, S ;

WEISSMAN, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (05) :1943-1947

[7] CONSTRUCTION OF A NORMALIZED CDNA LIBRARY BY INTRODUCTION OF A SEMISOLID MESSENGER-RNA-CDNA HYBRIDIZATION SYSTEM [J].

SASAKI, YF ;

AYUSAWA, D ;

OISHI, M .

NUCLEIC ACIDS RESEARCH, 1994, 22 (06) :987-992

[8] CONSTRUCTION AND CHARACTERIZATION OF A NORMALIZED CDNA LIBRARY [J].

SOARES, MB ;

BONALDO, MD ;

JELENE, P ;

SU, L ;

LAWTON, L ;

EFSTRATIADIS, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9228-9232

[9]

Staden R, 2000, Methods Mol Biol, V132, P115

[10]

VIEIRA J, 1987, METHOD ENZYMOL, V153, P3