Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins

被引：404

作者：

Hutchins, James R. A. ^{[2
]}

Toyoda, Yusuke ^{[1
]}

Hegemann, Bjoern ^{[2
]}

Poser, Ina ^{[1
]}

Heriche, Jean-Karim ^{[3
,4
]}

Sykora, Martina M. ^{[2
]}

Augsburg, Martina ^{[1
]}

Hudecz, Otto ^{[2
]}

Buschhorn, Bettina A. ^{[2
]}

Bulkescher, Jutta ^{[4
]}

Conrad, Christian ^{[4
]}

Comartin, David ^{[5
,6
]}

Schleiffer, Alexander ^{[2
]}

Sarov, Mihail ^{[1
]}

Pozniakovsky, Andrei ^{[1
]}

Slabicki, Mikolaj Michal ^{[1
]}

Schloissnig, Siegfried ^{[1
,7
]}

Steinmacher, Ines ^{[2
]}

Leuschner, Marit ^{[1
]}

Ssykor, Andrea ^{[1
]}

Lawo, Steffen ^{[5
,6
]}

Pelletier, Laurence ^{[5
,6
]}

Stark, Holger ^{[8
]}

Nasmyth, Kim ^{[2
]}

Ellenberg, Jan ^{[4
]}

Durbin, Richard ^{[3
]}

Buchholz, Frank ^{[1
]}

Mechtler, Karl ^{[2
]}

Hyman, Anthony A. ^{[1
]}

Peters, Jan-Michael ^{[2
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

[2] Res Inst Mol Pathol IMP, A-1030 Vienna, Austria

[3] Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England

[4] European Mol Biol Lab, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany

[5] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[6] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

[7] German Canc Res Ctr, D-69120 Heidelberg, Germany

[8] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

来源：

SCIENCE | 2010年 / 328卷 / 5978期

基金：

日本学术振兴会; 奥地利科学基金会;

关键词：

ANAPHASE-PROMOTING COMPLEX/CYCLOSOME; MAMMALIAN-CELL DIVISION; TISSUE-CULTURE CELLS; CAENORHABDITIS-ELEGANS; MICROTUBULE GENERATION; GLOBAL ANALYSIS; SPINDLE; GENOME; RNAI; CENTROSOME;

D O I：

10.1126/science.1181348

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate that the identity and composition of these protein complexes is incompletely understood. Using gene tagging on bacterial artificial chromosomes, protein localization, and tandem-affinity purification-mass spectrometry, the MitoCheck consortium has analyzed about 100 human protein complexes, many of which had not or had only incompletely been characterized. This work has led to the discovery of previously unknown, evolutionarily conserved subunits of the anaphase-promoting complex and the g-tubulin ring complex-large complexes that are essential for spindle assembly and chromosome segregation. The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells.

引用

页码：593 / 599

页数：7

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