Polypyrrole-polyvinyl sulphonate film based disposable nucleic acid biosensor

被引：61

作者：

Prabhakar, Nirmal

Arora, Kavita

Singh, Surinder P.

Pandey, Manoj K.

Singh, Harpal

Malhotra, Bansi D.

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Indian Inst Technol, Ctr Biomed Engn, New Delhi 110016, India

来源：

ANALYTICA CHIMICA ACTA | 2007年 / 589卷 / 01期

关键词：

nucleic acid biosensor; deoxyribose nucleic acid; polypyrrole-polyvinyl sulphonate; electrochemical entrapment; chlorpyrifos; malathion;

D O I：

10.1016/j.aca.2007.01.084

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Double stranded calf thymus deoxyribonucleic acid entrapped polypyrrole-polyvinyl sulphonate (dsCT-DNA-PPy-PVS) films fabricated onto indium-tin-oxide (ITO) coated glass plates have been used to detect organophosphates such as chlorpyrifos and malathion. These disposable dsCT-DNA-PPy-PVS/ITO bioelectrodes have been characterized using cyclic voltammetry, Fourier-transform-infra-red (FTIR) spectroscopy and atomic force microscopy (AFM), respectively. These biosensing electrodes have a response time of 30 s, are stable for about 5 months when stored in desiccated conditions at 25 degrees C and can be used to amperometrically detect chlorpyrifos (0.0016-0.025 ppm) and malathion (0.17-5.0), respectively. The additive effect of these pesticides on the amperometric response of the disposable dsCT-DNA-PPy-PVS/ITO bioelectrodes has also been investigated. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：6 / 13

页数：8

共 30 条

[1] Application of electrochemically prepared polypyrrole-polyvinyl sulphonate films to DNA biosensor [J].