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Pax7 is required for the specification of myogenic satellite cells

被引：1735
作者：
Seale, P
Sabourin, LA
Girgis-Gabardo, A
Mansouri, A
Gruss, P
Rudnicki, MA
机构：
[1] McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada
[2] McMaster Univ, Dept Pathol, Hamilton, ON L8S 4K1, Canada
[3] McMaster Univ, Inst Mol Biol & Biotechnol, Hamilton, ON L8S 4K1, Canada
[4] Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany
来源：
CELL | 2000年 / 102卷 / 06期
基金：
美国国家卫生研究院; 英国医学研究理事会; 加拿大自然科学与工程研究理事会;
关键词：
D O I：
10.1016/S0092-8674(00)00066-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The paired box transcription factor Pax7 was isolated by representational difference analysis as a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization revealed that Pax7 was also expressed in satellite cells residing in adult muscle. Cell culture and electron microscopic analysis revealed a complete absence of satellite cells in Pax7(-/-) skeletal muscle. Surprisingly, fluorescence-activated cell sorting analysis indicated that the proportion of muscle-derived stem cells was unaffected. Importantly, stem cells from Pax7(-/-) muscle displayed almost a 10-fold increase in their ability to form hematopoietic colonies. These results demonstrate that satellite cells and muscle-derived stem cells represent distinct cell populations. Together these studies suggest that induction of Pax7 in muscle-derived stem cells induces satellite cell specification by restricting alternate developmental programs.
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页码：777 / 786
页数：10
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