Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana

被引：58

作者：

Jun, L

Saiki, R

Tatsumi, K

Nakagawa, T

Kawamukai, M

机构：

[1] Shimane Univ, Fac Life & Environm Sci, Dept Appl Biosci & Biotechnol, Matsue, Shimane 6908504, Japan

[2] Shimane Univ, Ctr Integrated Res Sci, Matsue, Shimane 6908504, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2004年 / 45卷 / 12期

关键词：

coenzymeQ; solanesyl diphosphate synthase; ubiquinone;

D O I：

10.1093/pcp/pch211

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two solanesyl diphosphate synthases, designated SPS1 and SPS2, which are responsible for the synthesis of the isoprenoid side chain of either plastoquinone or ubiquinone in Arabidopsis thaliana, were identified. Heterologous expression of either SPS1 or SPS2 allowed the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli. SPS1-GFP was found to localize in the ER while SPS2-GFP localized in the plastid of tobacco BY-2 cells. These two different subcellular localizations are thought to be the reflection of their roles in solanesyl diphosphate synthesis in two different parts: presumably SPS1 and SPS2 for the side chains of ubiquinone and plastoquinone, respectively.

引用

页码：1882 / 1888

页数：7

共 28 条

[1] Molecular cloning of geranyl diphosphate synthase and compartmentation of monoterpene synthesis in plant cells [J].